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Akademie věd České republiky - Požadavky

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Výběrová řízení - Akademie věd České republikyThe Laboratory of Photosynthesis (Roman Sobotka’s group),Institute of Microbiology,Třeboň,Czech Republic,investigates the molecular principles of XXX assembly and maintenance of photosynthetic complexes in cyanobacteria (mainly Synechocystis PCC 6803).The group is currently participating in a large national project (Photomachines,2024-2028),focusing on the development of a novel type of photosynthetic chassis for biotechnological applications.A specific task of our research group is the design of light harvesting antennae for cyanobacteria,which will be based on chlorophyll and carotenoid cofactors and functionally similar to XXX XXXXX Harvesting Complexes of algae and plants.By using a small and pigment-rich antenna system,the original light harvesting complexes (phycobilisomes) in XXX cyanobacterium XXX be used as a large and rapidly degradable source of organic carbon and nitrogen molecules and directed towards a desired bioproduct <.> Candidates are invited to apply for a full-time Postdoc position starting XXXX XXXX Xnd XXXX for one-year contract,which XXX be extended for two more years in XXXX (three years in total) <.> The methodology of XXX project may include: In silico design,structure prediction and molecular dynamics of new antenna proteins for Synechocystis PCC 6803         Construction of Synechocystis mutants,characterization of their phenotypes,genome sequencing,heterologous expression Purification and analysis of membrane protein complexes – pulldown assays,2D gel systems,immunodetection,protein mass‐spectrometry Preparation of protein samples for Cryo-EM,analysis of Cryo-EM data HPLC and FPLC,various chromatographic techniques of proteins and pigments (ion‐exchange,gel‐filtration,reverse‐phase) Our requirements: PhD in natural sciences/life sciences (biochemistry,microbiology/plant biology,molecular biology,chemistry) Good knowledge of standard techniques in biochemistry,molecular biology and in bioi...

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